LOCUS SEGA076 4103 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 4103) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /codon_start=1 /label=glmS /note="glmS" /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" terminator 538..594 /label=L3S2P11 Terminator /note="L3S2P11 Terminator" misc_feature complement(611..1234) /label=TetR /note="TetR" /note="Geneious type: CDS" RBS 1235..1262 /label=tet1 /note="tet1" promoter complement(1263..1316) /label=pLacI /note="pLacI" /note="promoter" promoter 1343..1418 /label=pTet* /note="pTet*" misc_feature 1419..1493 /label=RiboJ /note="RiboJ" /note="insulator: self-cleaving ribozymes RiboJ" RBS 1505..1531 /note="TIR" /note="low TIR" CDS 1523..1578 /codon_start=1 /label=BCD /note="BCD" /translation="MKAIFVLKHLNHAKEVF" CDS 1579..2289 /codon_start=1 /product="green fluorescent protein" /label=GFP /note="GFP" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" terminator 2293..2341 /label=L3S3P22 Terminator (synthetic) /note="L3S3P22 Terminator" promoter 2352..2380 /label=Promoter 3BCD2 /note="Promoter 3BCD2" CDS 2420..3610 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="TcR" /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" terminator 3621..3661 /label=rrnC /note="rrnC" CDS 3856..4103 /codon_start=1 /label=pstS /note="pstS" /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccccaatt 601 aggatcctta tcaggaccca ctttcacatt taagttgttt ttctaatccg catatgatca 661 attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt 721 gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt 781 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat 841 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag 901 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac 961 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc 1021 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga 1081 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct 1141 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc 1201 tgttaatcac tttactttta tctaatctgg acatattcac caccctgaat tgactctctt 1261 ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggcg cgccgccatt 1321 ggagcttaac gatcgttggc tgttttcagc aggacgcact gacctcccta tcagtgatag 1381 agattgacat ccctatcagt gatagagata ctgagcacag ctgtcaccgg atgtgctttc 1441 cggtctgatg agtccgtgag gacgaaacag cctctacaaa taattttgtt taatactaga 1501 gattaaagag gagaaagggg ggatgaaggc tattttcgta ctgaaacatc ttaatcatgc 1561 taaggaggtt ttctaatgag caaaggagaa gaacttttca ctggagttgt gccaattctt 1621 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt 1681 gatgctacat acggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt 1741 ccatggccaa cacttgtcac tactctgacc tatggcgttc aatgcttttc ccgttatccg 1801 gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa 1861 cgcactatat ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt caagtttgaa 1921 ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga agatggaaac 1981 attctcggac acaaactaga gtacaactat aactcacaca atgtatacat cacggcagac 2041 aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacattga agatggttcc 2101 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta 2161 ccagacaacc attacctgtc gacacaatct gccctttcga aagatcccaa cgaaaagcgt 2221 gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag 2281 ctctacaaat gaccaattat tgaaggccgc taacgcggcc tttttttgtt tctggtctcc 2341 caaaaattta tttgcttatt aatcatccgg ctcgtataat gtgtggaggg cccaagttca 2401 cttaaaaagg agaatagtta tgaaatcgaa caatgcgctc atcgtcatcc tcggcaccgt 2461 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga 2521 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt 2581 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc 2641 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc 2701 cgtcctgtgg attctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc 2761 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg 2821 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt 2881 gggcgccatc tccctgcacg caccattcct tgcggcggcg gtgctcaacg gcctcaacct 2941 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgccgtc cgatgccctt 3001 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacca ttgtggccgc 3061 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 3121 cattttcggc gaggaccgct ttcgctggag cgcgacgatg agcggcctgt cgcttgcggt 3181 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 3241 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct 3301 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg 3361 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg 3421 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat 3481 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg 3541 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac 3601 ctcgacctga gacaattgtc cagataaaaa aaatccttag ctttcgctaa ggatgatttc 3661 taaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt 3721 atttaaatca gactgaagac tttatctctc tgtcataaaa ctgtcatatt ccttacatat 3781 aactgtcacc tgtttgtcct attttgcttc tcgtagccaa caaacaatgc tttatgaatc 3841 ctcccaggag acattatgaa agttatgcgt accaccgtcg caactgttgt cgccgcgacc 3901 ttatcgatga gtgctttctc tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc 3961 ttccctgcgc cggtgtatgc caaatgggct gacacttacc agaaagaaac cggtaataaa 4021 gttaactacc agggtatcgg ttcttccggt ggcgtaaaac agattatcgc taataccgtt 4081 gattttggtg cctctgacgc gcc //