LOCUS SEGA062 4413 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 4413) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /codon_start=1 /label=glmS /note="glmS" /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" terminator 538..594 /label=L3S2P11 Terminator /note="L3S2P11 Terminator" CDS complement(604..1686) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(1687..1764) /gene="lacI" /label=lacI promoter /note="lacI promoter" promoter 1795..1813 /label=T7 promoter /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 1814..1838 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 1850..1876 /note="TIR" /note="low TIR" CDS 1868..1888 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /note="TEV site" /translation="MENLYFQ" CDS 1889..2599 /codon_start=1 /product="green fluorescent protein" /label=GFP /note="GFP" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" terminator 2603..2651 /label=L3S3P22 Terminator (synthetic) /note="L3S3P22 Terminator" promoter 2662..2690 /label=Promoter 3BCD2 /note="Promoter 3BCD2" CDS 2730..3920 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="TcR" /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" terminator 3931..3971 /label=rrnC /note="rrnC" CDS 4166..4413 /codon_start=1 /label=pstS /note="pstS" /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 721 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 781 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 841 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 901 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 961 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 1021 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 1081 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 1141 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 1201 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 1261 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 1321 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 1381 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 1441 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 1501 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 1561 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 1621 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 1681 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 1741 ggttttgcgc cattcgatgg tgtcggatcg agatcgatct cgatcccgcg aaattaatac 1801 gactcactat aggggaattg tgagcggata acaattccta ctagagatta aagaggagaa 1861 acgggggatg gagaacctgt acttccagag caaaggagaa gaacttttca ctggagttgt 1921 gccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga 1981 gggtgaaggt gatgctacat acggaaaact cacccttaaa tttatttgca ctactggaaa 2041 actacctgtt ccatggccaa cacttgtcac tactctgacc tatggcgttc aatgcttttc 2101 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta 2161 tgtacaggaa cgcactatat ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt 2221 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga 2281 agatggaaac attctcggac acaaactaga gtacaactat aactcacaca atgtatacat 2341 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacattga 2401 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc 2461 tgtcctttta ccagacaacc attacctgtc gacacaatct gccctttcga aagatcccaa 2521 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg 2581 catggatgag ctctacaaat gaccaattat tgaaggccgc taacgcggcc tttttttgtt 2641 tctggtctcc caaaaattta tttgcttatt aatcatccgg ctcgtataat gtgtggaggg 2701 cccaagttca cttaaaaagg agaatagtta tgaaatcgaa caatgcgctc atcgtcatcc 2761 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc 2821 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc 2881 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg 2941 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg 3001 cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggccggc atcaccggcg 3061 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc 3121 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg 3181 ggggactgtt gggcgccatc tccctgcacg caccattcct tgcggcggcg gtgctcaacg 3241 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgccgtc 3301 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacca 3361 ttgtggccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag 3421 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg agcggcctgt 3481 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca 3541 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct 3601 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg 3661 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg 3721 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg 3781 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat 3841 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga 3901 gccgggccac ctcgacctga gacaattgtc cagataaaaa aaatccttag ctttcgctaa 3961 ggatgatttc taaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat 4021 atcaacgctt atttaaatca gactgaagac tttatctctc tgtcataaaa ctgtcatatt 4081 ccttacatat aactgtcacc tgtttgtcct attttgcttc tcgtagccaa caaacaatgc 4141 tttatgaatc ctcccaggag acattatgaa agttatgcgt accaccgtcg caactgttgt 4201 cgccgcgacc ttatcgatga gtgctttctc tgtgtttgca gaagcaagcc tgacaggtgc 4261 aggtgcaacc ttccctgcgc cggtgtatgc caaatgggct gacacttacc agaaagaaac 4321 cggtaataaa gttaactacc agggtatcgg ttcttccggt ggcgtaaaac agattatcgc 4381 taataccgtt gattttggtg cctctgacgc gcc //