LOCUS SEGA057 4448 bp ds-DNA linear UNA 08-SEP-2021 DEFINITION natural linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 4448) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /codon_start=1 /label=glmS /note="glmS" /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" terminator 538..594 /label=L3S2P11 Terminator /note="L3S2P11 Terminator" CDS complement(604..1686) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(1687..1764) /gene="lacI" /label=lacI promoter /note="lacI promoter" promoter 1795..1813 /label=T7 promoter /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 1814..1838 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 1850..1876 /note="TIR" /note="medium TIR" CDS 1868..1923 /codon_start=1 /label=BCD /note="BCD" /translation="MKAIFVLKHLNHAKEVF" CDS 1924..2634 /codon_start=1 /product="green fluorescent protein" /label=GFP /note="GFP" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" terminator 2638..2686 /label=L3S3P22 Terminator (synthetic) /note="L3S3P22 Terminator" promoter 2697..2725 /label=Promoter 3BCD2 /note="Promoter 3BCD2" CDS 2765..3955 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="TcR" /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" terminator 3966..4006 /label=rrnC /note="rrnC" CDS 4201..4448 /codon_start=1 /label=pstS /note="pstS" /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 721 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 781 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 841 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 901 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 961 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 1021 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 1081 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 1141 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 1201 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 1261 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 1321 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 1381 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 1441 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 1501 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 1561 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 1621 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 1681 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 1741 ggttttgcgc cattcgatgg tgtcggatcg agatcgatct cgatcccgcg aaattaatac 1801 gactcactat aggggaattg tgagcggata acaattccta ctagagatta aagaggagaa 1861 agagctaatg aaggctattt tcgtactgaa acatcttaat catgctaagg aggttttcta 1921 atgagcaaag gagaagaact tttcactgga gttgtgccaa ttcttgttga attagatggt 1981 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc tacatacgga 2041 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 2101 gtcactactc tgacctatgg cgttcaatgc ttttcccgtt atccggatca catgaaacgg 2161 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc 2221 aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt 2281 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct cggacacaaa 2341 ctagagtaca actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga 2401 atcaaagcta acttcaaaat tcgccacaac attgaagatg gttccgttca actagcagac 2461 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 2521 ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt 2581 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaatgacca 2641 attattgaag gccgctaacg cggccttttt ttgtttctgg tctcccaaaa atttatttgc 2701 ttattaatca tccggctcgt ataatgtgtg gagggcccaa gttcacttaa aaaggagaat 2761 agttatgaaa tcgaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt 2821 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga 2881 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg 2941 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc 3001 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggattct 3061 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 3121 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg 3181 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctccct 3241 gcacgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt 3301 cctaatgcag gagtcgcata agggagagcg ccgtccgatg cccttgagag ccttcaaccc 3361 agtcagctcc ttccggtggg cgcggggcat gaccattgtg gccgcactta tgactgtctt 3421 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga 3481 ccgctttcgc tggagcgcga cgatgagcgg cctgtcgctt gcggtattcg gaatcttgca 3541 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc 3601 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg 3661 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc 3721 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc 3781 gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta 3841 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct 3901 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgagacaa 3961 ttgtccagat aaaaaaaatc cttagctttc gctaaggatg atttctaaaa tttttctgtc 4021 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttattta aatcagactg 4081 aagactttat ctctctgtca taaaactgtc atattcctta catataactg tcacctgttt 4141 gtcctatttt gcttctcgta gccaacaaac aatgctttat gaatcctccc aggagacatt 4201 atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg cgaccttatc gatgagtgct 4261 ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg caaccttccc tgcgccggtg 4321 tatgccaaat gggctgacac ttaccagaaa gaaaccggta ataaagttaa ctaccagggt 4381 atcggttctt ccggtggcgt aaaacagatt atcgctaata ccgttgattt tggtgcctct 4441 gacgcgcc //