LOCUS SEGA050 5246 bp ds-DNA linear UNA 08-SEP-2021 DEFINITION natural linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 5246) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /codon_start=1 /label=glmS /note="glmS" /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" terminator 538..594 /label=L3S2P11 Terminator /note="L3S2P11 Terminator" CDS complement(607..1452) /codon_start=1 /gene="E. coli rhaR" /product="transcriptional activator of rhaR and rhaS" /label=rhaR /note="rhaR" /note="needed for rhamnose-dependent activation of the rhaB promoter" /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK D" CDS complement(1526..2362) /codon_start=1 /gene="E. coli rhaS" /product="positive regulator of the rhaB promoter" /label=rhaS /note="rhaS" /note="activated by L-rhamnose" /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ" promoter 2508..2626 /label=rhaB promoter /note="rhaB promoter" /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" RBS 2638..2664 /note="TIR" /note="low TIR" CDS 2656..2721 /codon_start=1 /gene="pelB (fragment)" /product="leader peptide for secretion" /label=pelB signal sequence /note="pelB signal sequence" /translation="MKYLLPTAAAGLLLLAAQPAMA" CDS 2722..3432 /codon_start=1 /product="superfolder GFP" /label=sfGFP /note="sfGFP" /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" terminator 3436..3484 /label=L3S3P22 Terminator (synthetic) /note="L3S3P22 Terminator" promoter 3495..3523 /label=Promoter 3BCD2 /note="Promoter 3BCD2" CDS 3563..4753 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="TcR" /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" terminator 4764..4804 /label=rrnC /note="rrnC" CDS 4999..5246 /codon_start=1 /label=pstS /note="pstS" /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgcttaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg 661 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact 721 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgtcgcag 781 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact 841 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag 901 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt 961 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt 1021 agcaaacggc acatgctgac tactttcatg ctcaagctga ccaataacct gccgcgcctg 1081 cgccatcccc atgctaccta agcgccagtg tggttgccct gcgctggcgt taaatcccgg 1141 aatcgccccc tgccagtcaa gattcagctt cagacgctcc gggcaataaa taatattctg 1201 caaaaccaga tcgttaacgg aagcgtagga gtgtttatca tcagcatgaa tgtaaaagag 1261 atcgccacgg gtaatgcgat aagggcgatc gttgagtaca tgcaggccat taccgcgcca 1321 gacaatcacc agctcacaaa aatcatgtgt atgttcagca aagacatctt gcggataacg 1381 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa 1441 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg 1501 tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat 1561 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga 1621 atccacagcg ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc agatgtcggg 1681 ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa 1741 gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca 1801 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt 1861 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga 1921 tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa 1981 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc 2041 catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg 2101 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat 2161 gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat 2221 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa 2281 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttaccagac ggaaaaaaat 2341 ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa 2401 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt 2461 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag 2521 caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca ttttcttgtc 2581 agtaacgaga aggtcgcgaa tccaggcgct ttttagactg gtcgtatact agagattaaa 2641 gaggagaaac ggacgatgaa gtacctgctg ccgaccgctg ctgctggtct gctgctcctc 2701 gctgcccagc cggcgatggc ccgtaaaggc gaagagctgt tcactggtgt cgtccctatt 2761 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa 2821 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg 2881 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat 2941 ccggaccaca tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag 3001 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt 3061 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc 3121 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc 3181 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc 3241 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg 3301 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa 3361 cgcgatcaca tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat 3421 gaactgtaca aatgaccaat tattgaaggc cgctaacgcg gccttttttt gtttctggtc 3481 tcccaaaaat ttatttgctt attaatcatc cggctcgtat aatgtgtgga gggcccaagt 3541 tcacttaaaa aggagaatag ttatgaaatc gaacaatgcg ctcatcgtca tcctcggcac 3601 cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg 3661 ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc 3721 gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg 3781 cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac 3841 acccgtcctg tggattctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg 3901 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt 3961 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact 4021 gttgggcgcc atctccctgc acgcaccatt ccttgcggcg gcggtgctca acggcctcaa 4081 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgcc gtccgatgcc 4141 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ccattgtggc 4201 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg 4261 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgagcggcc tgtcgcttgc 4321 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg 4381 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt 4441 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg 4501 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca 4561 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct 4621 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt 4681 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc 4741 cacctcgacc tgagacaatt gtccagataa aaaaaatcct tagctttcgc taaggatgat 4801 ttctaaaatt tttctgtcat ctcttcgtta ttaatgtttg taattgactg aatatcaacg 4861 cttatttaaa tcagactgaa gactttatct ctctgtcata aaactgtcat attccttaca 4921 tataactgtc acctgtttgt cctattttgc ttctcgtagc caacaaacaa tgctttatga 4981 atcctcccag gagacattat gaaagttatg cgtaccaccg tcgcaactgt tgtcgccgcg 5041 accttatcga tgagtgcttt ctctgtgttt gcagaagcaa gcctgacagg tgcaggtgca 5101 accttccctg cgccggtgta tgccaaatgg gctgacactt accagaaaga aaccggtaat 5161 aaagttaact accagggtat cggttcttcc ggtggcgtaa aacagattat cgctaatacc 5221 gttgattttg gtgcctctga cgcgcc //