LOCUS SEGA014 3249 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 3249) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /codon_start=1 /label=glmS /note="glmS" /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" terminator 538..594 /label=L3S2P11 Terminator /note="L3S2P11 Terminator" promoter 604..638 /label=P-J23100 /note="P-J23100" RBS 650..676 /note="TIR" /note="low TIR" CDS 668..688 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /note="TEV site" /translation="MENLYFQ" misc_feature 689..724 /label=PROTi-Linker /note="PROTi-Linker" CDS 725..1435 /codon_start=1 /product="green fluorescent protein" /label=GFP /note="GFP" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" terminator 1439..1487 /label=L3S3P22 Terminator (synthetic) /note="L3S3P22 Terminator" promoter 1498..1526 /label=Promoter 3BCD2 /note="Promoter 3BCD2" CDS 1566..2756 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="TcR" /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" terminator 2767..2807 /label=rrnC /note="rrnC" CDS 3002..3249 /codon_start=1 /label=pstS /note="pstS" /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgcttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta aagaggagaa 661 acgggggatg gagaacctgt acttccagtt tcgtagcaaa ggagaagaac ttgtcactgg 721 agttagcaaa ggagaagaac ttttcactgg agttgtgcca attcttgttg aattagatgg 781 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg ctacatacgg 841 aaaactcacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact 901 tgtcactact ctgacctatg gcgttcaatg cttttcccgt tatccggatc acatgaaacg 961 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt 1021 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt 1081 taatcgtatc gagttaaagg gtattgattt taaagaagat ggaaacattc tcggacacaa 1141 actagagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg 1201 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggttccgttc aactagcaga 1261 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta 1321 cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct 1381 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaatgacc 1441 aattattgaa ggccgctaac gcggcctttt tttgtttctg gtctcccaaa aatttatttg 1501 cttattaatc atccggctcg tataatgtgt ggagggccca agttcactta aaaaggagaa 1561 tagttatgaa atcgaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg 1621 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg 1681 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat 1741 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt 1801 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggattc 1861 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct 1921 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt 1981 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctccc 2041 tgcacgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct 2101 tcctaatgca ggagtcgcat aagggagagc gccgtccgat gcccttgaga gccttcaacc 2161 cagtcagctc cttccggtgg gcgcggggca tgaccattgt ggccgcactt atgactgtct 2221 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg 2281 accgctttcg ctggagcgcg acgatgagcg gcctgtcgct tgcggtattc ggaatcttgc 2341 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg 2401 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc 2461 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg 2521 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat 2581 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt 2641 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc 2701 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgagaca 2761 attgtccaga taaaaaaaat ccttagcttt cgctaaggat gatttctaaa atttttctgt 2821 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt aaatcagact 2881 gaagacttta tctctctgtc ataaaactgt catattcctt acatataact gtcacctgtt 2941 tgtcctattt tgcttctcgt agccaacaaa caatgcttta tgaatcctcc caggagacat 3001 tatgaaagtt atgcgtacca ccgtcgcaac tgttgtcgcc gcgaccttat cgatgagtgc 3061 tttctctgtg tttgcagaag caagcctgac aggtgcaggt gcaaccttcc ctgcgccggt 3121 gtatgccaaa tgggctgaca cttaccagaa agaaaccggt aataaagtta actaccaggg 3181 tatcggttct tccggtggcg taaaacagat tatcgctaat accgttgatt ttggtgcctc 3241 tgacgcgcc //