LOCUS SEGA012 3213 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 3213) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /codon_start=1 /label=glmS /note="glmS" /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" terminator 538..594 /label=L3S2P11 Terminator /note="L3S2P11 Terminator" promoter 604..638 /label=P-J23100 /note="P-J23100" RBS 650..676 /note="TIR" /note="medium TIR" CDS 668..688 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /note="TEV site" /translation="MENLYFQ" CDS 689..1399 /codon_start=1 /product="green fluorescent protein" /label=GFP /note="GFP" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" terminator 1403..1451 /label=L3S3P22 Terminator (synthetic) /note="L3S3P22 Terminator" promoter 1462..1490 /label=Promoter 3BCD2 /note="Promoter 3BCD2" CDS 1530..2720 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="TcR" /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" terminator 2731..2771 /label=rrnC /note="rrnC" CDS 2966..3213 /codon_start=1 /label=pstS /note="pstS" /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgcttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta aagaggagaa 661 aaactacatg gaaaacctgt acttccagag caaaggagaa gaacttttca ctggagttgt 721 gccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga 781 gggtgaaggt gatgctacat acggaaaact cacccttaaa tttatttgca ctactggaaa 841 actacctgtt ccatggccaa cacttgtcac tactctgacc tatggcgttc aatgcttttc 901 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta 961 tgtacaggaa cgcactatat ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt 1021 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga 1081 agatggaaac attctcggac acaaactaga gtacaactat aactcacaca atgtatacat 1141 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacattga 1201 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc 1261 tgtcctttta ccagacaacc attacctgtc gacacaatct gccctttcga aagatcccaa 1321 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg 1381 catggatgag ctctacaaat gaccaattat tgaaggccgc taacgcggcc tttttttgtt 1441 tctggtctcc caaaaattta tttgcttatt aatcatccgg ctcgtataat gtgtggaggg 1501 cccaagttca cttaaaaagg agaatagtta tgaaatcgaa caatgcgctc atcgtcatcc 1561 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc 1621 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc 1681 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg 1741 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg 1801 cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggccggc atcaccggcg 1861 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc 1921 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg 1981 ggggactgtt gggcgccatc tccctgcacg caccattcct tgcggcggcg gtgctcaacg 2041 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgccgtc 2101 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacca 2161 ttgtggccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag 2221 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg agcggcctgt 2281 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca 2341 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct 2401 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg 2461 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg 2521 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg 2581 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat 2641 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga 2701 gccgggccac ctcgacctga gacaattgtc cagataaaaa aaatccttag ctttcgctaa 2761 ggatgatttc taaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat 2821 atcaacgctt atttaaatca gactgaagac tttatctctc tgtcataaaa ctgtcatatt 2881 ccttacatat aactgtcacc tgtttgtcct attttgcttc tcgtagccaa caaacaatgc 2941 tttatgaatc ctcccaggag acattatgaa agttatgcgt accaccgtcg caactgttgt 3001 cgccgcgacc ttatcgatga gtgctttctc tgtgtttgca gaagcaagcc tgacaggtgc 3061 aggtgcaacc ttccctgcgc cggtgtatgc caaatgggct gacacttacc agaaagaaac 3121 cggtaataaa gttaactacc agggtatcgg ttcttccggt ggcgtaaaac agattatcgc 3181 taataccgtt gattttggtg cctctgacgc gcc //