LOCUS SEGA005 3248 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 3248) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /codon_start=1 /label=glmS /note="glmS" /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" terminator 538..594 /label=L3S2P11 Terminator /note="L3S2P11 Terminator" promoter 604..638 /label=P-J23100 /note="P-J23100" RBS 650..676 /note="TIR" /note="low TIR" CDS 668..723 /codon_start=1 /label=BCD /note="BCD" /translation="MKAIFVLKHLNHAKEVF" CDS 724..1434 /codon_start=1 /product="green fluoresent protein" /label=GFP /note="GFP" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" terminator 1438..1486 /label=L3S3P22 Terminator (synthetic) /note="L3S3P22 Terminator" promoter 1497..1525 /label=Promoter 3BCD2 /note="Promoter 3BCD2" CDS 1565..2755 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="TcR" /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" terminator 2766..2806 /label=rrnC /note="rrnC" CDS 3001..3248 /codon_start=1 /label=pstS /note="pstS" /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgcttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta aagaggagaa 661 aggggggatg aaggctattt tcgtactgaa acatcttaat catgctaagg aggttttcta 721 atgagcaaag gagaagaact tttcactgga gttgtgccaa ttcttgttga attagatggt 781 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc tacatacgga 841 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 901 gtcactactc tgacctatgg cgttcaatgc ttttcccgtt atccggatca catgaaacgg 961 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc 1021 aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt 1081 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct cggacacaaa 1141 ctagagtaca actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga 1201 atcaaagcta acttcaaaat tcgccacaac attgaagatg gttccgttca actagcagac 1261 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 1321 ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt 1381 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaatgacca 1441 attattgaag gccgctaacg cggccttttt ttgtttctgg tctcccaaaa atttatttgc 1501 ttattaatca tccggctcgt ataatgtgtg gagggcccaa gttcacttaa aaaggagaat 1561 agttatgaaa tcgaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt 1621 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga 1681 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg 1741 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc 1801 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggattct 1861 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 1921 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg 1981 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctccct 2041 gcacgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt 2101 cctaatgcag gagtcgcata agggagagcg ccgtccgatg cccttgagag ccttcaaccc 2161 agtcagctcc ttccggtggg cgcggggcat gaccattgtg gccgcactta tgactgtctt 2221 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga 2281 ccgctttcgc tggagcgcga cgatgagcgg cctgtcgctt gcggtattcg gaatcttgca 2341 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc 2401 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg 2461 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc 2521 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc 2581 gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta 2641 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct 2701 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgagacaa 2761 ttgtccagat aaaaaaaatc cttagctttc gctaaggatg atttctaaaa tttttctgtc 2821 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttattta aatcagactg 2881 aagactttat ctctctgtca taaaactgtc atattcctta catataactg tcacctgttt 2941 gtcctatttt gcttctcgta gccaacaaac aatgctttat gaatcctccc aggagacatt 3001 atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg cgaccttatc gatgagtgct 3061 ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg caaccttccc tgcgccggtg 3121 tatgccaaat gggctgacac ttaccagaaa gaaaccggta ataaagttaa ctaccagggt 3181 atcggttctt ccggtggcgt aaaacagatt atcgctaata ccgttgattt tggtgcctct 3241 gacgcgcc //