SEGA is a standardized genome engineering platform that allows for predictable expression levels and rapid interchange of functional modules. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.
In its simplest form, SEGA enables genome engineering by combining only two reagents: a DNA fragment that can be ordered from a commercial vendor and a stock solution of bacterial cells, followed by incubation on agar plates.
Chromosomal integration is often favoured over plasmid-based expression. Plasmids have inherent disadvantages like constraints on the size and the use of antibiotics. With SEGA, genetic elements are integrated into the chromosome and no antibiotics need to be supplemented to maintain stable strains.
The standardized design of SEGA facilitates the integration of a gene-of-interest with high efficiency and predictable expression control across the genome.